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Effects of GANT61 on ALK + ALCL cell proliferation: a At 0 h, 24 h, 48 h, and 72 h following treatment with varying concentrations of GANT61, cell viability of the Karpas-299 cell line were assessed. b At 0 h, 24 h, 48 h, and 72 h after exposure to different concentrations of GANT61, survival rates of the SU-DHL-1 cell line were analyzed (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Journal: Annals of Hematology

Article Title: GANT61 suppresses proliferation and induces apoptosis in ALK-Positive anaplastic large cell lymphoma via modulating the Hh-PIK3IP1-Akt signaling axis

doi: 10.1007/s00277-026-06827-2

Figure Lengend Snippet: Effects of GANT61 on ALK + ALCL cell proliferation: a At 0 h, 24 h, 48 h, and 72 h following treatment with varying concentrations of GANT61, cell viability of the Karpas-299 cell line were assessed. b At 0 h, 24 h, 48 h, and 72 h after exposure to different concentrations of GANT61, survival rates of the SU-DHL-1 cell line were analyzed (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: Gli1 inhibitor GANT61 and DMSO were obtained from MCE (USA).

Techniques:

Effects of GANT61 on cell cycle distribution in ALK + ALCL: a Cell cycle distribution of the Karpas-299 cell line following 48-hour exposure to varying concentrations of GANT61. b At 0 h, 12 h, 24 h, and 48 h after treatment with 12 µM GANT61, cell cycle distribution of the Karpas-299 cell line was profiled. c Cell cycle distribution of the SU-DHL-1 cell line after 48-hour treatment with different concentrations of GANT61. (d) At 0 h, 12 h, 24 h, and 48 h after treatment with 12 µM GANT61, cell cycle distribution of the SU-DHL-1 cell line was profiled (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Journal: Annals of Hematology

Article Title: GANT61 suppresses proliferation and induces apoptosis in ALK-Positive anaplastic large cell lymphoma via modulating the Hh-PIK3IP1-Akt signaling axis

doi: 10.1007/s00277-026-06827-2

Figure Lengend Snippet: Effects of GANT61 on cell cycle distribution in ALK + ALCL: a Cell cycle distribution of the Karpas-299 cell line following 48-hour exposure to varying concentrations of GANT61. b At 0 h, 12 h, 24 h, and 48 h after treatment with 12 µM GANT61, cell cycle distribution of the Karpas-299 cell line was profiled. c Cell cycle distribution of the SU-DHL-1 cell line after 48-hour treatment with different concentrations of GANT61. (d) At 0 h, 12 h, 24 h, and 48 h after treatment with 12 µM GANT61, cell cycle distribution of the SU-DHL-1 cell line was profiled (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: Gli1 inhibitor GANT61 and DMSO were obtained from MCE (USA).

Techniques:

GANT61 promoted apoptosis in the Karpas-299 cell line: a , b Following 12 h, 24 h, and 48 h treatment with GANT61 dose gradients, apoptotic cell percentages in Karpas-299 were determined via flow cytometry. c , d Effects of different concentrations of GANT61 on apoptotic molecules in the Karpas-299 cell line. e , f Time-dependent effects of 12 µM GANT61 on apoptotic molecules in the Karpas-299 cell line at designated time points (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Journal: Annals of Hematology

Article Title: GANT61 suppresses proliferation and induces apoptosis in ALK-Positive anaplastic large cell lymphoma via modulating the Hh-PIK3IP1-Akt signaling axis

doi: 10.1007/s00277-026-06827-2

Figure Lengend Snippet: GANT61 promoted apoptosis in the Karpas-299 cell line: a , b Following 12 h, 24 h, and 48 h treatment with GANT61 dose gradients, apoptotic cell percentages in Karpas-299 were determined via flow cytometry. c , d Effects of different concentrations of GANT61 on apoptotic molecules in the Karpas-299 cell line. e , f Time-dependent effects of 12 µM GANT61 on apoptotic molecules in the Karpas-299 cell line at designated time points (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: Gli1 inhibitor GANT61 and DMSO were obtained from MCE (USA).

Techniques: Flow Cytometry

GANT61 promoted apoptosis in the SU-DHL-1 cell line: a , b Following 12 h, 24 h, and 48 h treatment with GANT61 dose gradients, apoptotic cell percentages in SU-DHL-1 cultures were determined via flow cytometry. c , d Effects of different concentrations of GANT61 on apoptotic molecules in the SU-DHL-1 cell line. e , f Time-dependent effects of 12 µM GANT61 on apoptotic molecules in the SU-DHL-1 cell line at designated time points (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Journal: Annals of Hematology

Article Title: GANT61 suppresses proliferation and induces apoptosis in ALK-Positive anaplastic large cell lymphoma via modulating the Hh-PIK3IP1-Akt signaling axis

doi: 10.1007/s00277-026-06827-2

Figure Lengend Snippet: GANT61 promoted apoptosis in the SU-DHL-1 cell line: a , b Following 12 h, 24 h, and 48 h treatment with GANT61 dose gradients, apoptotic cell percentages in SU-DHL-1 cultures were determined via flow cytometry. c , d Effects of different concentrations of GANT61 on apoptotic molecules in the SU-DHL-1 cell line. e , f Time-dependent effects of 12 µM GANT61 on apoptotic molecules in the SU-DHL-1 cell line at designated time points (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001)

Article Snippet: Gli1 inhibitor GANT61 and DMSO were obtained from MCE (USA).

Techniques: Flow Cytometry

GANT61 disrupted the PI3K/Akt signaling pathway in ALK + ALCL cell lines: a , b , d , e Protein expression levels of Gli1, PIK3IP1, p-Akt, and Akt were evaluated for GANT61-mediated effects using western blot analysis in ALK + ALCL cell lines. c , f Regulation of Gli1 , PIK3IP1 , and Akt mRNA levels by GANT61 in ALK + ALCL cell lines (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Journal: Annals of Hematology

Article Title: GANT61 suppresses proliferation and induces apoptosis in ALK-Positive anaplastic large cell lymphoma via modulating the Hh-PIK3IP1-Akt signaling axis

doi: 10.1007/s00277-026-06827-2

Figure Lengend Snippet: GANT61 disrupted the PI3K/Akt signaling pathway in ALK + ALCL cell lines: a , b , d , e Protein expression levels of Gli1, PIK3IP1, p-Akt, and Akt were evaluated for GANT61-mediated effects using western blot analysis in ALK + ALCL cell lines. c , f Regulation of Gli1 , PIK3IP1 , and Akt mRNA levels by GANT61 in ALK + ALCL cell lines (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Gli1 inhibitor GANT61 and DMSO were obtained from MCE (USA).

Techniques: Expressing, Western Blot